The rapid spread of the disease in affected animals generates significant economic losses worldwide.
DAS DEV KEYGEN VIRUS FREE
More than 100 countries are not yet recognized as officially free of FMD by the World Organisation for Animal Health (OIE). The ability of strip tests to produce rapid results and high specificity makes it a valuable tool for early detection of FMDV O, A and Asia 1 in the field.įoot-and-mouth disease (FMD) remains one of the world’s most widespread epizootic and highly contagious animal diseases. All of them demonstrated positive results using the LFI serotype A strip test and double antibody sandwich (DAS) ELISA. Swab samples (n=11) collected over the lesion area from experimentally inoculated animals (serotype A) were examined. All vesicular fluid and epithelium samples collected from experimentally infected animals with serotype O, A and Asia 1 were identified as positive by the LFI strip test. The sensitivity of this strip test was comparable with the double antibody sandwich ELISA for viral antigen detection.
Whereas the test for serotype O detected 45 out of 46 field isolates.
The LFI strip tests for serotypes A and Asia 1 were able to identify all tested serotype A (n= 39) and Asia 1 field isolates (n=17). ResultĮach strip test can specifically detect the FMDV O, A or Asia 1 viruses, but not other vesicular disease viruses. A new generation of the generic RapidAssay Device (gRAD) was used for the test. A serotype independent mAb was selected and used as the detection mAb with the aim of subsequently developing a multi-serotype strip test. Specific monoclonal antibodies (mAbs) against each serotype were produced and used as the capture mAbs. The purpose of this project is to develop a lateral flow immunochromatographic (LFI) strip test for rapid detection of FMD virus serotypes O, A and Asia 1. Thus, the development of a rapid and easily performed test for FMD detection is important for controlling a FMD outbreak and containing its spread. Outbreaks of Foot-and-mouth disease (FMD) have resulted in tremendous economic losses.